pollen

Birch and grass pollen grains as well as pollen-derived small particles appear as potent allergens in the outdoor air during spring and summer. The occurrence of pollen allergens in indoor air, however, has not been studied in depth due to lack of suitable sampling and analytical methods. Herein; a recently reported "direct on sampling filter estimation" (DOSAFE) technique (Acevedo et al., 1998) has been validated for quantification of pollen allergens in indoor air using two school rooms and two office rooms as experimental models.